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pDNA Quanta Mega Kit (In <40 Minutes)

MDI Quanta Mega Kit is uniquely designed to facilitate ultrapure pDNA (2.5 mg) free from transfection even into highly sensitive mammalian cells. These kits offer high yields from both low copy as well as high copy number plasmids. The innovative buffer system and filter device provides efficient binding of pDNA onto the spin column.

Washing is done with the help of provided wash buffers in order to remove endotoxins, RNA, proteins and polysaccharides. The technology also does away with hasselsome gravitational waiting, phenol extraction (associated with desalting) and ethanol precipitation (associated with anion exchange based purification).

  • Fastest Megaprep for pDNA Isolation
  • Very high binding capacity – Upto 3000µg
  • No gravitational waiting
  • Simple filtration to remove cell debris – No centrifugation
  • No precipitation required for concentration
  • Higher yields with low culture volume
  • Highly concentrated, ultrapure pDNA isolation in very low elution volumes
  • High within lot as well as lot to lot consistency

  • Automated Fluorescent Sequencing
  • Radioactive Sequencing
  • Restriction Digestion
  • Transfection (with highly sensitive mammalian cell lines)
  • Cloning
  • PCR

Specifications
Plasmid Type
High Copy Plasmid Low Copy Plasmid
Capacity of Tube Extender
300 ml 300 ml
Binding Capacity of Spin Column
3000 µg 3000 µg
Maximum culture volume
500 ml 500 ml
Expected Yield of Plasmid
2500 µg 2500 µg
Assurance
Ultrapure Plasmid DNA A260 / A280 = 1.8-2.0
Quality Management System ISO-9001 Certified

Type
Quanta Mega Kit QMPK
XX
XX
XX
X
Pack Size
Pack Size Code
2 0002
10 0010

Example :

QMPK XX XX XX X 0010

File Type Download
User Manual Download

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