Express 96 PCR Purification Kit
mdi Express 96 PCR Purification Kit is a fast, economical and easy way to purify DNA fragments (100bp-10kb) from PCR amplification reactions. The buffer system provided in the kit allows selective binding of DNA fragments from PCR reactions to the Express prep plate.
Washing is done with the help of provided wash buffer in order to remove primers, nucleotides, enzymes, mineral oil, salts and other impurities from DNA samples. Purified DNA is eluted in low salt buffer or water for a variety of downstream applications. The technology does away with the cumbersome methodologies of phenol extraction (associated with slurries formation) as well as ethanol precipitation (associated with anion exchange based purification system) for desalting.
- Multiple (96) samples processing in just 45 minutes
- Very high binding capacity
- No precipitation required for desalting
- Highly concentrated, ultrapure pDNA isolation in very low elution volumes
- High within lot as well as lot to lot consistency
- Ligation
- Transformation/ Transduction/ Transfection
- Automated Fluorescent Sequencing
- Radioactive Sequencing
- Restriction Digestion
- Labeling
- Cloning
| Specifications | |
| Binding capacity of membrane (ds DNA) | 10µg / Well |
| Recovered DNA fragment | (100 bp - 10 kb) |
| Minimum Elution Volume | 100µl |
| Total eluate volume | 60µl |
| Capacity of a well | 800µl |
| Recovery of DNA | 80-95% |
| Assurance | |
| Ultrapure Plasmid DNA | A260 / A280 = 1.8-2.0 |
| Quality Management System | ISO-9001 Certified |
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Example :
| EPCK | XX | XX | XX | X | 0004 |
| User Manual | Download |
