-800x500.jpg "PCR Purification Kit (5-10μg)")
PCR Purification Kit (5-10μg)
mdi PCR Purification Kit is a fast and economical way to purify DNA fragments (100bp-10Kb) from PCR amplification reactions in small elution volume.
The mdi PCR purification system combines specially developed mdi membrane, reagents and the convenient spin column technology to capture target DNA. The contaminants such as enzymes, primers, nucleotides etc. are washed away and the bound target DNA is eluted in a low salt concentration elution buffer.
- Easy purification of large sized fragments without shearing
- No precipitation step for high purity DNA
- Highly concentrated DNA yields in low elution volume
- Transfection
- In-Vitro Transcription
- In-Vitro Translation
- High quality sequencing
- Microarray Analysis
- Cloning
- PCR
- Gene Silencing
- Probe Generation
- Microinjection
- Restriction Digestion
- Demanding enzymatic modifications
- Library Construction
- Bacterial Transformation
- Ligation
| Specifications | |
| Binding capacity of membrane (ds DNA) | 10 μg |
| Recovered DNA fragment | (100 bp -10 kb) |
| Capacity of column reservoir | 800μl |
| Minimum Elution Volume | 30 μl |
| Total eluate volume | 28 μl |
| Recovery | 90-95% |
| Assurance | |
| Ultrapure Plasmid DNA | A260 / A280 = 1.8-2.0 |
| Quality Management System | ISO-9001 Certified |
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Example :
| SPCK | XX | XX | XX | X | 0250 |
