The reliability of the method used for the isolation/purification of
pDNA is of critical importance when considering the success of
subsequent downstream molecular applications.
The mdi
Miniprep system makes use of the ability of the unique mdi membrane
that binds up to 25µg of pDNA along with the convenient spin column
technology. The bound pDNA is eluted in small volumes of very low salt
concentration buffer.
This technology does away with time
consuming Phenol-Chloroform extraction and alcohol precipitation. The
highly pure eluted pDNA is suitable for PCR, cloning, ligation, probe
generation, microinjection, transformation/transfection, restriction
digestion, radioactive sequencing and automated fluorescent sequencing.