Genomic DNA Miniprep Kit
The MDI Genomic DNA Miniprep Kit is a fast, economical and easy isolation method of high purity gDNA from animal (mammalian) tissues, blood and bacterial cells. The buffer system provided in the kit allows cell lysis followed by selective binding of gDNA to the spin column.
Purified gDNA is eluted in low-salt buffer or water for variety of downstream applications. This technology also does away with the cumbersome methodologies of phenol extraction. (associated with slurries formation) as well as ethanol precipitation (associated with anion exchange based purification system) for desalting.
- Very high binding capacity – Up to 50μg
- Higher yields
- No precipitation step for high purity gDNA
- Suitable for all type of downstream applications
- PCR
- Southern Blotting
- RAPD Analysis
- AFLP Analysis
- RFLP Analysis
- In- Vitro Transcription
- Restriction Digestion
- Transformation
- Transfection
- SNP Genotyping
- Microsatellite Analysis
- RT – PCR
- Gene Silencing
- Microinjection
- Probe Generation
| Specifications | |
| Maximum Amount of Tissue | 25 mg |
| Maximum amount of cultured cells | 5 x 106 |
| Maximum bacterial cells | 2×109 |
| Maximum volume of blood sample | 200μl |
| Binding capacity of membrane (ds DNA) | 50 μg |
| Capacity of column reservoir | 700μl |
| Minimum Elution Volume | 200μl |
| Recovery | 80% |
| Assurance | |
| Ultrapure Plasmid DNA | A260 / A280 = 1.8-2.0 |
| Quality Management System | ISO-9001 Certified |
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Example :
| CTGK | XX | XX | XX | X | 0250 |
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